블로그

Osteoporosis (OP) is a systemic bone disease characterized by decreased bone mass and bone tissue microstructure damage, which leads to increased bone fragility and susceptibility to fractures. This disease is highly prevalent among the elderly and is common in the spine and hip. Among them, vertebral compression fractures caused by osteoporosis are particularly common, and can even be life-threatening in severe cases. Current treatment options are costly and may cause adverse stimulation to the intestines, liver, and kidneys, increasing the risk of other diseases. Therefore, researchers are working hard to develop alternative drugs with better effects, fewer side effects, and lower costs.

In a study, researchers fed osteoporosis experimental group mice with traditional Chinese medicine to evaluate its therapeutic effect. To ensure the accuracy and reliability of the data, a grinder was used to pre-treat the samples during the study to obtain homogenized and high-quality samples. This treatment method helps to extract key biological indicators and reduce experimental errors. After a course of treatment, the researchers compared in detail the changes in gene expression, sex hormone levels, and bone density of the experimental group and model group mice, aiming to comprehensively evaluate the potential efficacy of traditional Chinese medicine treatment on osteoporosis.

In many laboratories, grinding is an important step in sample pretreatment, especially in the biomedical field. By processing samples with a grinder, tissue samples can be homogenized to ensure that the components in the sample are fully released, providing high-quality samples for subsequent analysis. In osteoporosis research, the grinding process helps to extract genes, proteins and other biomolecules from mouse bone tissue, thereby ensuring the accuracy and repeatability of experimental data. In addition, grinding can also reduce errors in sample processing and improve the reliability of experimental results. Next, we will introduce in detail the operation method, precautions and specific applications of the grinder in experiments.

Experimental Equipment:

Welso cryo-grinding machine

The cryo grinder is a fast, efficient, and consistent system for multiple test tubes that can efficiently process samples from a variety of sources, including soil, plant and animal tissues/organs, bacteria, yeast, fungi, spores, and paleontological specimens. It can be used to extract and purify raw DNA, RNA, and proteins to ensure the integrity and stability of the samples. The cryo grinder has a wide range of applications in life sciences, medicine, biochemistry, environmental science, geology, agriculture, forestry, quality inspection, and materials science. Its efficient processing capabilities and consistency make it an indispensable tool in modern scientific research and industry, providing reliable support for sample analysis and research.

Experimental purpose

The rib tissues of the experimental and control mice were ground and nucleic acids were extracted to analyze gene expression. The rib samples were homogenized by a cryo-grinder, which can efficiently extract high-quality DNA and RNA, providing reliable samples for subsequent gene expression analysis. The extracted nucleic acid samples will be used for molecular biology techniques such as quantitative PCR and RNA sequencing to compare the differences in gene expression between the experimental and control groups, so as to further explore the potential mechanism of action of traditional Chinese medicine on osteoporosis.

Experimental materials and instruments

Materials: mouse ribs, grinding tubes, grinding beads

Instrument: cryo-grinder

Experimental steps

Prepare 2 mL wear-resistant grinding tubes and a set of metal adapters.

Put the rib samples into the grinding tubes and soak them with liquid nitrogen.

After the samples are completely frozen, put the grinding tubes into the cryo-grinder, set the appropriate grinding parameters, and start grinding.

After grinding, take out the samples for further analysis.

Experimental results:

After grinding the mouse ribs with a cryo-grinder, the samples were in a uniform powder state. Liquid nitrogen cryo-grinder can effectively protect the activity of nucleic acids, providing an ideal sample for subsequent nucleic acid extraction and purification. The extracted nucleic acid samples will be used for gene expression analysis, comparing the differences in gene expression between the experimental group and the control group, in order to further explore the mechanism of the effect of traditional Chinese medicine on osteoporosis.