In molecular biology research, efficient grinding of yeast liquid is crucial for protein extraction. Traditional methods often result in incomplete cell disruption or heat-induced protein inactivation, compromising experimental accuracy. A cryo mill offers an ideal solution, utilizing liquid nitrogen freezing combined with high-speed vibration to effectively break cell walls while preserving protein activity. This ensures enhanced grinding efficiency and reliable experimental results.
Whether for precise grinding of small sample batches or efficient high-throughput processing, the cryo mill delivers stable and reliable performance. Its exceptional grinding uniformity and repeatability enhance experimental efficiency while ensuring data accuracy and reliability, empowering researchers to overcome experimental challenges and achieve superior research outcomes.
Advantages of the Cryo Mill
Precise Temperature Control in Ultra-Low Environments
The intelligent system maintains a stable -196℃ environment, preventing heat-induced protein inactivation and preserving sample activity throughout the grinding process.
Innovative 3D Grinding Technology
Advanced 3D grinding ensures uniform sample crushing and rapid processing, significantly enhancing grinding efficiency and performance.
Boosted Protein Extraction Rate
Experimental results show the cryo mill increases protein extraction rates by over 90% compared to traditional methods, ensuring higher experimental success and reliable data.
Efficient and Accurate Data Assurance
The cryo mill’s high performance and precision guarantee sample consistency and integrity, laying a strong foundation for subsequent analysis.
Versatile for Varied Experimental Needs
From small-batch precision grinding to high-throughput applications, the cryo mill adapts to diverse requirements, optimizing experimental efficiency and flexibility.
Preparation of Yeast Samples with Cryo Mill
Experimental Purpose:
This experiment aims to utilize a cryo mill for efficient grinding of yeast liquid to break cell walls while preserving protein activity, providing a solid foundation for subsequent protein extraction. By leveraging an ultra-low temperature environment and advanced 3D grinding technology, the method ensures complete cell disruption while preventing heat-induced protein inactivation. The results will evaluate the method's effectiveness in enhancing protein extraction efficiency and maintaining protein integrity, delivering high-quality samples for further experimental analysis.
Experimental Steps:
Material Preparation
Prepare a 2mL*24 adapter and the required yeast liquid samples. Ensure that all experimental equipment, grinding beads, and adapters are clean and contamination-free.
Sample Loading
Add an appropriate amount of yeast liquid to a 2mL centrifuge tube, along with an equal volume of mixed grinding beads (0.5mm and 1mm). Ensure the ratio of grinding beads to the sample meets experimental requirements, then securely cap the centrifuge tube.
Adapter Setup
Place the centrifuge tube into the corresponding hole of the 2mL×24 adapter. Ensure the groove at the bottom of the adapter locks firmly into the shaft slot. Adjust the adapter to a horizontal position and place the centrifuge tubes symmetrically. Cover the adapter with the lid and tighten the nut securely.
Set Grinding Parameters
Configure the grinding machine to a frequency of 70Hz and a grinding time of 60 seconds, with a 10-second pause. Perform 5-8 cycles, adjusting the time and cycles as needed based on the number of samples and desired grinding results.
Grinding Process
Start the grinder to initiate the grinding process. Pause and adjust the grinding time if necessary to ensure complete cell disruption.
Final Check
After grinding, inspect the samples to confirm thorough cell disruption before proceeding with subsequent steps.
This method efficiently disrupts yeast liquid samples, preserves protein activity, and provides high-quality samples for subsequent analysis.
Experimental Results:
The image above demonstrates the grinding effect of the Welso Cryo Mill. The sample is fully ground with excellent results, effectively replacing traditional manual grinding methods. The Cryo Mill’s built-in refrigeration system efficiently absorbs heat during grinding, preventing sample degradation from temperature rise and preserving protein activity. Capable of processing 24 samples at once, it significantly enhances the efficiency of traditional manual methods. Through extensive comparisons and evaluations, the Cryo Mill has proven to improve experimental efficiency and reliability, making it an ideal alternative to manual grinding.
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