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Blood is made up of blood cells and plasma, and blood cells are made up of red blood cells, white blood cells and platelets, no anticoagulants are added, centrifugation separates light yellow liquid which is called serum.

Serum contains active substances such as proteins, enzymes, amino acids, vitamins, etc., which are mainly used for immune assessment and disease diagnosis.

Whole blood samples are needed to be pre-treated and separating serum by using a low-speed centrifuge. This is currently the most efficient method. So how to isolate serum correctly?

Materials and Instruments

● Whole blood samples

● Disposable Pipette tips

● Micro tube 1.5mL

● Centrifuge tube racks

● Aseptic operation table

● Low-speed centrifuge

● Micro Pipettes

Experimental Procedure

Collect blood samples, put them into the centrifuge tube, gently mix them 5 or 6 times.No violent shaking here in order to avoid hemolysis, and leave it at room temperature for about 15 minutes, the blood in the tube will be coagulated and does not flow.

Place the centrifuge tube containing the whole blood sample into the centrifuge and set speed to 3000-3500 rpm for 5-10 min.

After centrifugation, use a micro pipette and pipette tips to slowly transfer the serum to a sterile centrifuge tube.

Storage of serum requires a further temperature of -20°C or lower, which is able to maintain stability and antigenic activity.

Precautions

●The whole process of operation shall be under sterile environment to avoid contamination of experimental samples

●The speed and time of the centrifuge need to be determined according to samples

●After collecting the blood, separate serum and plasma as soon as possible to avoid hemolysis, and repeated freezing and thawing of serum and plasma.

●Blood centrifugation speed should not be too fast, as too fast centrifugation can cause hematocrit and hemolysis.

●If the blood sample cannot be separated from serum or plasma immediately, it should be stored and transported as refrigerated as possible, and should never be frozen. Otherwise, the blood cells will form ice crystals, causing the cells to rupture and cause hemolysis.

Low-speed centrifuge is one of the necessary instruments in clinical medicine, biology, immunology and other fields. Mainly is benchtop model with a speed not exceed 10000rpm. Simple structure and convenient operation with horizontal rotor and tube rack which can realize  multiple uses, is a cost-effective equipment.

The Welso Low-speed Centrifuge Has The Following Features:

● User friendly interface, simple operation, high-sensitivity touch screen, can be operated with gloves.

● Brushless motor drive, stable operation and lower noise

● With double locking function, door lock safety detection

● All parts in contact with the sample are made of 304 stainless steel, which is anti-corrosion and durable

●It has protection functions such as door lid closing, wrong operation, overspeed, over temperature, unbalance, etc., which is safe and reliable;

●The lifting speed can be adjusted quickly and slowly to prevent the sample from being suspended and ensure the centrifugation effect.